Towards on-site testing of Phytophthora species

Autor:	Stephan König, Sabine Werres, Matthias Urban, Jürgen Popp, Karina Weber, Dana Cialla-May, Lydia Schwenkbier, Sibyll Pollok
Rok vydání:	2015
Předmět:	General Chemical Engineering DNA–DNA hybridization Site testing General Engineering Loop-mediated isothermal amplification Biology biology.organism_classification Rapid detection Silver nanoparticle Analytical Chemistry Botany Phytophthora Target gene Biological system
Zdroj:	Analytical Methods. 7:211-217
ISSN:	1759-9679 1759-9660
DOI:	10.1039/c4ay02287d
Popis:	Rapid detection and accurate identification of plant pathogens in the field is an ongoing challenge. In this study, we report for the first time on the development of a helicase-dependent isothermal amplification (HDA) in combination with on-chip hybridization for the detection of selected Phytophthora species. The HDA approach allows efficient amplification of the yeast GTP-binding protein (Ypt1) target gene region at one constant temperature in a miniaturized heating device. The assay's specificity was determined by on-chip DNA hybridization and subsequent silver nanoparticle deposition. The silver deposits serve as stable endpoint signals that enable the visual as well as the electrical readout. Our promising results point to the direction of a near future on-site application of the combined techniques for a reliable detection of Phytophthora species.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7e7a60054fa124fe5d07e44bfd13df96 https://doi.org/10.1039/c4ay02287d Zobrazit plný text záznamu