Development of a New Amycolatopsis orientalis Strain for High-Yield Eremomycin Production
| Autor: | Konstantin G. Skryabin, V. Yu. Balabanyan, V. V. Dzhavakhiya, M. G. Chernobrovkin, D. L. Shobolov, E. D. Popova, E. V. Glagoleva, A. I. Ovchinnikov |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
food.ingredient biology Strain (chemistry) Mutant biology.organism_classification Applied Microbiology and Biotechnology Biochemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology food chemistry Yield (chemistry) Skimmed milk Glycerol Composition (visual arts) Fermentation Food science Amycolatopsis orientalis |
| Zdroj: | Applied Biochemistry and Microbiology. 53:792-799 |
| ISSN: | 1608-3024 0003-6838 |
| DOI: | 10.1134/s0003683817080026 |
| Popis: | A multistep random UV mutagenesis of an eremomycin-producing strain Amycolatopsis orientalis VKPM Ac-1125 with rather low productivity (0.5 g/L) and the further selection of the most high-yield mutant strains have resulted in a highly productive E 13-26 strain. After optimization of the fermentation medium by multifactor analysis, the most efficient composition for the fermentation medium was established, %: glycerol, 8; soybean meals, 2.0; MgCl2, 0.02; KH2PO4, 0.02; KNO3, 0.6; CaCl2, 0.3; skim milk powder, 0.46; and glucose, 0.8. The maximum productivity of this strain on the optimized medium reached 3.42 ± 0.17 g/L. Therefore, the obtained strain is considered to be very promising for further use in the selection and development of technology for the large-scale eremomycin production. |
| Databáze: | OpenAIRE |
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