| Autor: |
Isabel Spencer-Martins, Bärbel Hahn-Hägerdal, César Fonseca, Helena Rodrigues de Sousa, Rute I. S. Romão |
| Rok vydání: |
2007 |
| Předmět: |
|
| Zdroj: |
The FEBS Journal. 274:3589-3600 |
| ISSN: |
1742-464X |
| DOI: |
10.1111/j.1742-4658.2007.05892.x |
| Popis: |
Two yeasts, Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012, which show rapid growth on L-arabinose and very high rates of L-arabinose uptake on screening, were selected for characterization of L-arabinose transport and the first steps of intracellular L-arabinose metabolism. The kinetics of L-arabinose uptake revealed at least two transport systems with distinct substrate affinities, specificities, functional mechanisms and regulatory properties. The L-arabinose catabolic pathway proposed for filamentous fungi also seems to operate in the yeasts studied. The kinetic parameters of the initial L-arabinose-metabolizing enzymes were determined. Reductases were found to be mostly NADPH-dependent, whereas NAD was the preferred cofactor of dehydrogenases. The differences found between the two yeasts agree with the higher efficiency of L-arabinose metabolism in C. arabinofermentans. This is the first full account of the initial steps of L-arabinose catabolism in yeast including the biochemical characterization of a specific L-arabinose transporter. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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