An acid-labile linker for solid-phase oligoribonucleotide synthesis using Fmoc group for 5′-hydroxyl protection

Autor:	ElMostafa Alazzouzi, Enrique Pedroso, Fernando Gordillo, Anna Grandas, Yolanda Palom
Rok vydání:	1993
Předmět:	Ribonucleotide Pyrimidine Stereochemistry Organic Chemistry Acetal Ribonucleoside Biochemistry chemistry.chemical_compound chemistry Drug Discovery Polymer chemistry Peptide bond Piperidine Protecting group Linker
Zdroj:	Tetrahedron Letters. 34:2195-2198
ISSN:	0040-4039
DOI:	10.1016/s0040-4039(00)60380-5
Popis:	An alkoxybenzylidene acetal linker formed with the 2′- and 3′-OH of the 3′-terminal ribonucleoside and attached to the solid support through an amide bond fulfills the necessary requirements of base stability and acid lability to be used in solid phase oligoribonucleotide synthesis in combination with Fmoc and Mthp groups for 5′-OH and 2′-OH protection respectively. Methyl protected phosphates do not give rise to N-methylation of pyrimidine bases (T or U) when piperidine, instead of DBU, is used to remove the Fmoc groups.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7e463bd8278cbaaeb3f4d837c3c9f1cb https://doi.org/10.1016/s0040-4039(00)60380-5 Zobrazit plný text záznamu