Characterization of TLR4/MD-2-modified Au sensor surfaces towards the detection of molecular signatures of bacteria
| Autor: | Nora W. C. Chan, Kaveh Amini, Heinz-Bernhard Kraatz, Iraklii I. Ebralidze |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
biology General Chemical Engineering technology industry and agriculture General Engineering Nanotechnology 02 engineering and technology Quartz crystal microbalance 010402 general chemistry 021001 nanoscience & nanotechnology Electrochemistry biology.organism_classification 01 natural sciences 0104 chemical sciences Analytical Chemistry Characterization (materials science) Transducer X-ray photoelectron spectroscopy lipids (amino acids peptides and proteins) 0210 nano-technology Biosensor Bacteria Volume concentration |
| Zdroj: | Analytical Methods. 8:7623-7631 |
| ISSN: | 1759-9679 1759-9660 |
| Popis: | Lipopolysaccharides (LPSs), also known as endotoxins, can be fatal even at low concentrations. As a result, the development of novel methodologies for LPS detection has been continuously in the focus of research. Biosensors, which employ a bio-recognition element on a transducer surface, are on the cutting edge of these novel technologies. In this report, Au surfaces modified with TLR4/MD-2 through Lip-NHS linkers with an ultimate potential application as biosensors for LPS detection have been characterized and investigated using X-ray photoelectron spectroscopy, quartz crystal microbalance and electrochemical techniques. Also the interaction between TLR4/MD-2 immobilized on Au surfaces with LPSs has been studied to evaluate the possibility of LPS detection. |
| Databáze: | OpenAIRE |
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