Genome-wide identification and expression analyses of homeodomain-leucine zipper family genes reveal their involvement in stress response in apple (Malus × domestica)
Autor: | Yu-Jin Hao, Kai-Di Gu, Quan-Yan Zhang, Tao Chen, Jia-Hui Wang, Xun Wang, Da-Gang Hu, Jian-Qiang Yu |
---|---|
Rok vydání: | 2022 |
Předmět: |
0106 biological sciences
0301 basic medicine Genetics Leucine zipper Malus Ecology biology Renewable Energy Sustainability and the Environment fungi Plant Science biology.organism_classification 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Genome 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Arabidopsis Structural motif Gene Abscisic acid Ecology Evolution Behavior and Systematics Function (biology) 010606 plant biology & botany |
Zdroj: | Horticultural Plant Journal. 8:261-278 |
ISSN: | 2468-0141 |
Popis: | The homeodomain-leucine zipper (HD-Zip) family has been shown to perform a multitude of functions during plant development and stress responses; however, the family members and functions have not been identified in apple (Malus × domestica). In this study, 83 HD-Zips (MdHDZs) were identified in the apple genome. They were assembled into four subgroups according to the classification in Arabidopsis, where MdHDZs in the same subgroup had similar gene structures and conserved protein motifs. Putative cis-element analysis of MdHDZs promoter regions uncovered numerous elements related to the response of stress and plant hormones. In addition, twelve transcripts of the MdHDZs showed different expression patterns under salt, drought, low temperature and ABA stresses by quantitative reverse transcription-PCR (qRT-PCR) assay. To further explore the function of MdHDZs in apple, MdHDZ3 was selected to verify its function under salt, low temperature and ABA stresses; and genetic transformation was used to obtain MdHDZ3 transgenic apple calli. The results demonstrated that MdHDZ3 increased sensitivity to salt, low temperature and abscisic acid in apple calli, suggesting that MdHDZ3 plays an important role in response to stresses. Subcellular localization and three-dimensional structural analysis revealed that MdHDZ3 was a nuclear-localized protein. Taken together, these findings provide potential information for further identification of HD-Zip proteins in apple. |
Databáze: | OpenAIRE |
Externí odkaz: |