Tomographic imagery by interference microscopy

Autor:	Gael Moneron, Claude Boccara, Arnaud Dubois, Kate Grieve, Wilfrid Schwartz, Elvire Guiot
Rok vydání:	2005
Předmět:	Physics Microscope medicine.diagnostic_test Optical sectioning business.industry Linnik interferometer Interference microscopy Coherence length law.invention Interferometry Optics Optical coherence tomography law Microscopy medicine business
Zdroj:	SPIE Proceedings.
ISSN:	0277-786X
DOI:	10.1117/12.586727
Popis:	We have developed a white-light interference microscope as an alternative technique to conventional optical coherence tomography (OCT). The experimental setup is based on a Linnik interferometer illuminated with a tungsten halogen lamp. En face tomographic images are obtained in real-time without scanning by computing the difference of two phase-opposed interferometric images recorded by a CCD camera. The short coherence length of the source yields an optical sectioning ability with 0.7 μm resolution (in water). Transverse resolution of 0.9 μm is achieved by using high numerical aperture microscope objectives. A shot-noise limited detection sensitivity of 90 dB can be reached with ~ 1 s acquisition time. High-resolution images of mouse and tadpole embryos are shown.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7e182168f0d38d5645d1be3931e51222 https://doi.org/10.1117/12.586727 Zobrazit plný text záznamu