Effects of ingestion of a green seaweed,Ulva lactuca, upon caecal and colonic mucosas in the germ-free rat and in the heteroxenic rat harbouring a human bacterial flora

Autor: Anne Hibert, Claude Andrieux, Odette Szylit, Jean-Claude Meslin, Martine Bensaada
Rok vydání: 1999
Předmět:
Zdroj: Journal of the Science of Food and Agriculture. 79:727-732
ISSN: 1097-0010
0022-5142
DOI: 10.1002/(sici)1097-0010(199904)79:5<727::aid-jsfa243>3.0.co;2-o
Popis: Effect of ingestion of green seaweed, Ulva lactuca, (70 g kg−1) during a 6-week period on caecal and colonic mucosas was studied in germ-free (GF) rats and in heteroxenic (HE) rats harbouring a human bacterial flora (GF rats associated with a human flora). The pH and sulphide concentration of the caecal contents, crypt morphometry, mitotic index and mucin types in the caecal and distal colonic mucosas were determined. In the GF caecum, Ulva strongly increased crypt depth and mucin-containing cells irrespective of the mucin type studied (neutral, acidic or sulphated) compared to the control diet but had no significant effect on mitotic index. The crypt depth and mucin-containing cells in the caecum were higher in HE than in GF control rats. They were slightly but significantly increased by Ulva. In the distal colon mucosa of GF rats, Ulva decreased crypt depth and cell number as well as sulphomucin-containing cells. Conversely, in the HE rats, it increased crypt depth and reduced the number of neutral mucin-containing cells. These results show that Ulva lactuca exerts an intrinsic effect on mucosal morphometry and on mucin biosynthesis in GF rats. No pathological alteration was observed in the mucosas and no significant modification of the mitotic index or sulphide production was observed in HE rats. © 1999 Society of Chemical Industry
Databáze: OpenAIRE
Externí odkaz: