Phytochelatin Concentrations and Binding State of Cd in Roots of Maize Genotypes Differing in Shoot/Root Cd Partitioning

Autor: M.L. Van Beusichem, J.A. De Knecht, P. J. Florijn
Rok vydání: 1993
Předmět:
Zdroj: Journal of Plant Physiology. 142:537-542
ISSN: 0176-1617
DOI: 10.1016/s0176-1617(11)80395-0
Popis: Summary To investigate Cd binding in the root system as a possible cause of differential Cd distribution in maize inbred lines, Cd present in ‹soluble› and ‹insoluble pools› in roots of a ‹shoot Cd excluder› (B73) and a ‹non-shoot Cd excluder› (H98) was determined after extraction of the roots in HEPES buffer solution (pH 6.5). At a similar root Cd concentration (approximately 70 μg Cdg-1dry wt.), about 49 % of the Cd concentration in roots of H98 but 25 % of that in roots of B73 was removed during extraction (‹soluble pooh›). The acid-soluble thiol concentrations in roots of the ‹shoot Cd excluder› B73 were much lower compared with those of the ‹non-shoot Cd excluder› H98. Exposure of B73 and H98 plants to the same Cd concentration in solution resulted in nearly equal acid-soluble thiol concentrations in the root extracts. Binding characteristics of Cd to structural components of the roots (‹insoluble pool›) were similar for both maize inbreds. The relationship between transport of Cd from roots to shoots and amounts of Cd complexed to acid-soluble thiol compounds in the ‹soluble pool› of ‹non-shoot Cd excluders› compared with ‹shoot Cd excluders› is discussed.
Databáze: OpenAIRE