Fluorescence sensing of glucose using glucose oxidase incorporated into a fluorophore-containing PNIPAM hydrogel
| Autor: | Jongho Kim, Geunseok Jang, Daigeun Kim, Yongkyun Kim, Taek Seung Lee |
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| Rok vydání: | 2016 |
| Předmět: |
Fluorophore
Aqueous solution Polymers and Plastics biology Metal ions in aqueous solution Organic Chemistry Bioengineering 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Fluorescence 0104 chemical sciences chemistry.chemical_compound chemistry Rhodamine B biology.protein Organic chemistry Glucose oxidase Fluorescein 0210 nano-technology Hydrogen peroxide Nuclear chemistry |
| Zdroj: | Polymer Chemistry. 7:1907-1912 |
| ISSN: | 1759-9962 1759-9954 |
| DOI: | 10.1039/c5py02026c |
| Popis: | We present a new composite material composed of pH sensitive fluorescent dyes in a poly(N-isopropylacrylamide)-based hydrogel and incorporating glucose oxidase (GOx), which provides a platform for fluorescence sensing of glucose in the clinically relevant range of 100 μM to 100 mM in aqueous solution. Fluorescein and rhodamine B dyes are well known for their dramatic pH-responsive changes in fluorescence emission color, in which they were introduced to the hydrogel as comonomers. As D-glucose was oxidized to D-glucono-δ-lactone producing hydrogen peroxide and a proton through the enzymatic action of GOx, the generated proton affects the fluorescence of the composite hydrogel. The composite hydrogel exhibited high selectivity to glucose over other sugars and metal ions. The combined role of a soft hydrogel and oxidase highlights the potential of the new composite material design for selective detection of glucose in aqueous solution. |
| Databáze: | OpenAIRE |
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