Electrophoretic karyotype of Flammulina velutipes and its variation among monokaryotic progenies

Autor:	Makoto Ogawa, Motonobu Yoshida, Yurie Kitamura, Kenjiro Kinugawa, Kan Okabe, Eiji Tanesaka
Rok vydání:	2003
Předmět:	Genetics Gel electrophoresis biology Homogeneous Chromosome Karyotype biology.organism_classification Ribosomal DNA Molecular biology Ecology Evolution Behavior and Systematics Dikaryon Flammulina Electrophoretic karyotype
Zdroj:	Mycoscience. 44:67-69
ISSN:	1340-3540
Popis:	The karyotype of Flammulina velutipes (Curt. : Fr.) Sing. was investigated using contour-clamped homogeneous electric fields (CHEF) gel electrophoresis. A parental dikaryotic stock, JA, was resolved into at least eight chromosomal DNA bands ranging from 1.4- to 4.9-megabase (Mb) pairs. Overall, little size variation was found among monokaryotic strains with a few major exceptions. Among 13 monokaryotic progenies examined, 11 strains were resolved into at least eight chromosomal DNA bands in a manner similar to the parent dikaryon, whereas the other 2 were resolved into at least seven chromosomes lacking the 2.1-Mb chromosome possessed in the former. A slightly larger size variation was found in a chromosome carrying ribosomal DNA. An estimated haploid genome size of this stock was 24.0Mb or more.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7dd38d990eed09b7f0e9c740ed02e278 https://doi.org/10.1007/s10267-002-0082-z Zobrazit plný text záznamu Full text from SpringerLink