An Analytical System for Rapid Separation of Tissue Nucleotides at Low Pressures on Conventional Anion Exchangers

Autor:	Joseph X. Khym
Rok vydání:	1975
Předmět:	chemistry.chemical_classification Chromatography Aqueous solution Biochemistry (medical) Clinical Biochemistry Ion chromatography Inorganic chemistry Polymer Solvent chemistry.chemical_compound Column chromatography chemistry Nucleotide Amine gas treating Perchloric acid
Zdroj:	Clinical Chemistry. 21:1245-1252
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/21.9.1245
Popis:	An analytical anion-exchange procedure has been developed for the rapid separation of acid-soluble nucleotides (the so-called "free" or tissue nucleotides). It permits assay at low pressures (275-415 kPa; 40-60 psi) in less than 1 h on 10-cm columns of Aminex resins (conventional styrene-type anion exchangers) with alkaline citrate solutions as the eluent. Separation variables have been investigated, to determine optimum conditions for the routine analysis of samples containing tissue nucleotides. Also described here is a simple solvent-extraction procedure for removing HClO4 or CCl3CO2H quantitatively from cell extracts that contain acid-soluble nucleotides: they are removed from aqueous acid solutions with a water-insoluble amine dissolved in a water-immiscible solvent.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7dc7e106353e1e719663a5559519b0c8 https://doi.org/10.1093/clinchem/21.9.1245 Zobrazit plný text záznamu