Production and Characterization of Murine Single Chain Fv Antibodies to Aflatoxin B 1 Derived From a Pre-immunized Antibody Phage Display Library System
| Autor: | Anthony J. Killard, Bernadette M. Manning, Stephen J. Daly, Paul P. Dillon, Lynsey Dunne, Richard O'Kennedy |
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| Rok vydání: | 2002 |
| Předmět: |
Aflatoxin
Phage display biology Chemistry Immunology technology industry and agriculture biology.organism_classification Molecular biology law.invention Bacteriophage chemistry.chemical_compound law Recombinant DNA biology.protein Antibody Surface plasmon resonance Mycotoxin Agronomy and Crop Science Biosensor Food Science |
| Zdroj: | Food and Agricultural Immunology. 14:255-274 |
| ISSN: | 1465-3443 0954-0105 |
| DOI: | 10.1080/0954010021000096373 |
| Popis: | The production and characterization of recombinant antibodies to aflatoxin B 1 (AFB 1 ), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor (BIAcore), which measures biomolecular interactions in 'real-time'. Single chain Fv (scFv) antibodies were generated to aflatoxin B 1 from an established phage display system, which incorporated a range of different plasmids for efficient scFv expression. The scFv's were used in the development of a competitive ELISA, and also for the development of surface plasmon resonance (SPR)-based inhibition immunoassays. They were found to be suitable for the detection of AFB 1 , in this format, with the assays being sensitive and reproducible. |
| Databáze: | OpenAIRE |
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