| Autor: |
Laura Tarnawski, Zhengbing Zhuge, Eric J. Kort, Eddie Weitzberg, Shavva Vs, Alessandro L. Gallina, April S. Caravaca, Mika Gustafsson, Kehr J, Martinez Enguita D, Wang F, Schmidt S, Michael Eberhardson, Stefan Jovinge, Peder S. Olofsson, Matthew Weiland, Anna Färnert, Henrik Hult, Stephen Malin, Mattias Carlström |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.1101/2021.04.27.441632 |
| Popis: |
Vasodilation is a cornerstone of inflammation physiology. By regulating vasodilation and tissue entry of T cells, CD4+ T lymphocytes expressing choline acetyltransferase (ChAT), a key enzyme for biosynthesis of the vasorelaxant acetylcholine (ACh), critically link immunity with vascular biology in mice. However, the characterization of primary human ChAT+ T cells remained elusive. Here, we identified human ChAT+ T cells and report that ChAT mRNA was induced by activation. Functional studies demonstrated that T cell-derived ACh increased muscarinic ACh-receptor dependent NO-synthase activity and vasorelaxation. Further, single-cell RNA-sequencing revealed ChAT+CD4+ T cells in blood from patients with severe circulatory failure and a high relative frequency of ChAT+CD4+ T cells correlated with better 30-day survival in this cohort. Our findings provide the first insights into ChAT biology in primary human T cells, linking ChAT+ T cells with vasorelaxation as well as survival in a cohort of critically ill patients. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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