Supplementation with β-carotene or vitamin E protects against increase in anaphylactic response in β-lactoglobulin-sensitized Balb/c mice: ex vivo study
| Autor: | Leila Amier, H. Grar, Djamel Saidi, Samira Bekkaddour Benattia, Kamel Eddine El Mecherfi, Omar Kheroua, H. Negaoui, H. Kaddouri, H. Gourine, W. Dib |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
biology Ussing chamber Chemistry Vitamin E medicine.medical_treatment Carotene General Chemistry Immunoglobulin E biology.organism_classification Biochemistry Industrial and Manufacturing Engineering BALB/c Jejunum medicine.anatomical_structure Endocrinology Internal medicine Immunology biology.protein medicine Ex vivo Sensitization Food Science Biotechnology |
| Zdroj: | European Food Research and Technology. 241:393-398 |
| ISSN: | 1438-2385 1438-2377 |
| DOI: | 10.1007/s00217-015-2471-5 |
| Popis: | Balb/c mice fed 0 mg, 20 mg of β-carotene (BC) or 50 mg of vitamin E (Vit E) per Kg of diet for 7 weeks were immunized intraperitoneally with β-Lactoglobulin (β-Lg). Specific IgG and IgE anti β-Lg were determined in the mouse sera by an enzyme-linked immunosorbent assay (ELISA), and the jejunum was used for assessment of local anaphylactic responses ex vivo in Ussing chambers. Short-circuit current (Isc), a measure of active ion transport, and epithelial conductance (G), a measure of passive ion permeability, were measured for each tissue. Specimens were also processed for examination by histological analysis. Compared with sensitized mice (unsupplemented mice), those fed BC or Vit E had lower β-Lg-specific IgG (p |
| Databáze: | OpenAIRE |
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