Immunomodulating properties of protein fractions isolated from Delphinium staphysagria seeds
| Autor: | Rachid Bencheikh, Ouarda Azdad, Mohamed El Kabbaoui, Lotfi Aarab, Najlae Mejrhit, Amal Bousfiha, Alae Chda, Abdelali Tazi |
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| Rok vydání: | 2016 |
| Předmět: |
Pharmacology
Hemagglutination biology 010405 organic chemistry Chemistry Phagocytosis Antigen presentation Pharmaceutical Science Plant Science biology.organism_classification 01 natural sciences Molecular biology 0104 chemical sciences 010404 medicinal & biomolecular chemistry Complementary and alternative medicine Delphinium Lectin pathway Drug Discovery Splenocyte Cytotoxic T cell MTT assay |
| Zdroj: | Journal of Medicinal Plants Research. 10:29-34 |
| ISSN: | 1996-0875 |
| DOI: | 10.5897/jmpr2015.5957 |
| Popis: | The aim of this study was to investigate the immunomodulatory activities of protein fractions isolated from Delphinium staphysagria seeds used in Moroccan traditional medicine. D. staphysagria protein extract has been separated on sephadex column chromatography. The immunomodulatory activities were tested using primary culture of rabbit lymphocytes (splenocytes and thymocytes), by evaluation of macrophage phagocytosis and by studying the hemagglutination effect of proteins separated by chromatography. Three protein fractions were isolated on sephadex column. At 0.1mg/ml, these fractions didn’t show any cytotoxic effect against lymphocytes. The fraction F1 with the high molecular weight of 115 kDa exhibited a mitogenic action on splenocytes and positive hemagglutinating activity associated to inhibition of macrophage phagocytosis capacity. The second protein fraction F2 was about 11 kDa inhibited phagocytosis without affecting lymphocytes proliferation. The third fraction F3 was the smallest with 2.25 kDa indicated a stimulatory effect on both splenocytes and thymocytes proliferation without hemagglutinating activity but inducing decrease of macrophage phagocytosis. In conclusion, protein fractions isolated from D. staphysagria have no cytotoxic effect. The fraction F3 acted as an immunostimulating by enhancing proliferation of thymocytes and splenocytes while the F1 stimulated preferentially splenocytes via probably lectin pathway. All fractions studied decreased phagocytosis impairing the antigen presentation to lymphocytes. Key words: Delphinium staphysagria, protein fractions, immunomodulation, mitogenic effect, hemagglutination, MTT assay. |
| Databáze: | OpenAIRE |
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