Transcriptional and Translational Regulation of Angiotensin II Type 2 Receptor by Angiotensin II and Growth Factors1
| Autor: | O. Avallet, José M. Saez, Marie Claude Berthelon, Dominique Langlois, J Yuan Li |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
endocrine system
Messenger RNA medicine.medical_specialty Chemistry Growth factor medicine.medical_treatment Basic fibroblast growth factor respiratory system Molecular biology Angiotensin II chemistry.chemical_compound Endocrinology Cell culture Internal medicine Translational regulation cardiovascular system medicine Receptor hormones hormone substitutes and hormone antagonists circulatory and respiratory physiology Transforming growth factor |
| Zdroj: | Endocrinology. 140:4988-4994 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.140.11.7089 |
| Popis: | The regulatory effects of angiotensin-II (AngII) and several growth factors, including insulin-like growth factor 1 (IGF-1), basic fibroblast growth factor (bFGF), and transforming growth factor β1 (TGFβ1) on the AngII subtype 2 (AT2) receptor were studied using R3T3 cells, a mouse fibroblast cell line that expresses only AT2 receptors. AngII increased (in a time- and dose-dependent manner) AT2 binding sites but had no effects on AT2 messenger RNA (mRNA) levels. At maximal concentration (10−7 m) AngII caused a 4-fold increase of AT2 receptor number. In contrast, IGF-1 increased (3- to 4-fold), whereas bFGF and TGFβ1 decreased (by about 90% and 80%, respectively) AT2 receptor and mRNA levels. Moreover, AngII potentiated the effect of IGF-1 on receptor number, but not on AT2 mRNA levels, and significantly reduced the inhibitory action of bFGF and TGFβ1 on AT2 binding sites but not on AT2 mRNA levels. None of these factors modified AT2 mRNA half-life. The potential effects of these factors on transcription o... |
| Databáze: | OpenAIRE |
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