Autor:	C. Alexander Boecker
Rok vydání:	2022
DOI:	10.17504/protocols.io.81wgby723vpk/v1
Popis:	This protocol describes the preparation and culture of mouse primary cortical neurons for live-imaging experiments. Cortices were dissected from mouse embryos at day 15.5. Cortical neurons were isolated by digestion with 0.25% Trypsin and trituration with a serological pipette. Neurons were plated on glass-bottom imaging dishes in Attachment Media. After 5 hours in culture, Attachment Media was replaced with Maintenance Media, and AraC was added on the next day to prevent glia cell proliferation. Neurons were transfected 16-24 hours before imaging using Lipofectamine 2000.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7d514dbd9044dfcc27bb295be46b9431 https://doi.org/10.17504/protocols.io.81wgby723vpk/v1 Zobrazit plný text záznamu