Molecular cloning and anti-fungal effect of endo-β-1,3-glucanase from Thermotoga maritima
| Autor: | Hae Na Kang, Chang Bin Woo, Soo Bok Lee |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Curdlan Biology Glucanase Molecular cloning medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Microbiology Laminarin chemistry.chemical_compound Enzyme chemistry Biochemistry Thermotoga maritima medicine bacteria Candida albicans Escherichia coli Food Science Biotechnology |
| Zdroj: | Food Science and Biotechnology. 23:1243-1246 |
| ISSN: | 2092-6456 1226-7708 |
| DOI: | 10.1007/s10068-014-0170-9 |
| Popis: | A gene encoding an endo-β-1,3-glucanase from Thermotoga maritima MSB8 (TmβG) was cloned and expressed in Escherichia coli. The purified enzyme produced various β-1,3-glucooligosaccharides from soluble laminarin, and mainly β-1,3-glucooligosaccharides smaller than laminaritetrose from insoluble curdlan. The optimum pH and temperature of the enzyme were 5.0 and 80°C, respectively. TmβG inhibited the growth of Candida albicans, which indicates that the enzyme could potentially be used as an anti-fungal agent to control invasive infections. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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