Abrogation of Immunologic Unresponsiveness by Whole Molecules and Fragments of Cross-Reacting Immunoglobulins
| Autor: | R. W. Scales, J. M. Cruse |
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| Rok vydání: | 1970 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 105:1072-1081 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.105.5.1072 |
| Popis: | A state of immunologic tolerance to a given antigen can be terminated by the injection of a cross-reacting antigen provided that the degree of cross-reactivity is within certain limits. The present investigation was designed to test the ability of human γG or its fragments to terminate tolerance to bovine γG or its fragments and vice versa. γG of both species was isolated by DEAE-cellulose chromatography and digested by mercuripapain. C57BL mice were injected with 1.0 mg of tolerogen and 20 days later were immunized with 0.2 mg of the cross-reacting antigen in Freund's incomplete adjuvant. Seven days after the last immunizing injection, 125I-tolerogen was injected intraperitoneally and the course of elimination followed by whole body counting. Sera were assayed for antibodies to the tolerogen by gel diffusion, immunoelectrophoresis and passive hemagglutination. Results of this investigation demonstrated that tolerance to bovine γG in mice may be terminated by injection of human γG or its fragments. Likewise, tolerance to human γG in mice may be terminated by injection of bovine γG or its fragments. |
| Databáze: | OpenAIRE |
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