Change inLimnaea stagnalis neuronal response to acetylcholine during intracellular and extracellular perfusion with serotonin

Autor: O. P. Yurchenko, T. M. Turpaev, N. G. Grigor'ev
Rok vydání: 1987
Předmět:
Zdroj: Neurophysiology. 18:238-243
ISSN: 1573-9007
0090-2977
DOI: 10.1007/bf01052542
Popis: The modifying effect of adding serotonin to the intra- and extracellular environment on the inward currents generated by the cell following intracellular application of acetylcholine was shown during studies on unidentified isolatedLimnaea stagnalis neurons using techniques of intracellular perfusion and voltage clamping. Serotonin inhibited response to achetylcholine in both cases in most of the test neurons. Serotonin intensified this response when applied to the intracellular environment and produced the opposite effect of reducing the amplitude of inward acetylcholine currents when administered extracellularly. Cyproheptadine, the serotonin receptor blocker, inhibited the enhancing effect of serotonin produced by adding this neurotransmitter to the intracellular fluid, but mimicked the inhibitory effects of serotonin on response to acetylcholine, whether added to the intra- or extracellular environment. Findings would suggest the presence of intracellular serotonin receptors in the mollusk neurons; one of their possible functions could be controlling the sensitivity of the cell surface cholinoreceptors.
Databáze: OpenAIRE
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