ChemInform Abstract: Covalent Modification and Surface Immobilization of Nucleic Acids via the Diels-Alder Bioconjugation Method

Autor:	Dominick J. Anziano, Michael Leuck, David P. Sebesta, Gregory M. Husar
Rok vydání:	2010
Předmět:	Bioconjugation Chemistry Oligonucleotide Covalent bond Immobilized Nucleic Acids Reagent Nucleic acid Diels alder Covalent modification General Medicine Combinatorial chemistry
Zdroj:	ChemInform. 32
ISSN:	1522-2667 0931-7597
DOI:	10.1002/chin.200147209
Popis:	The importance of chemically modified and surface immobilized nucleic acids has inspired the development of a wide variety of complementary techniques for covalent oligonucleotide preparation and immobilization. We are developing technology based on the use of a Diels-Alder reaction for accomplishing the covalent modification of oligonucleotides. Reported herein is preliminary progress toward the establishment of robust reagents for introducing the reactive functionality, as well as studies employing the BIACORE system to demonstrate surface immobilization by the method.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7d1e2ea00164cdb45c15c2fc3c1a1ff7 https://doi.org/10.1002/chin.200147209 Zobrazit plný text záznamu Plný text