Comparison of the Antioxidant and Cytotoxic Activities of Phyllanthus virgatus and Phyllanthus amarus Extracts
| Autor: | Nuannoi Chudapongse, Kanchana Poompachee |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Phyllanthus
Antioxidant Traditional medicine biology business.industry Linoleic acid medicine.medical_treatment General Medicine biology.organism_classification law.invention chemistry.chemical_compound Biochemistry chemistry law Respiration medicine Cytotoxic T cell Trypan blue Cytotoxicity business Clark electrode |
| Zdroj: | Medical Principles and Practice. 21:24-29 |
| ISSN: | 1423-0151 1011-7571 |
| Popis: | Objective: To determine the antioxidant activity and cytotoxicity of Phyllanthus virgatus crude extract compared to Phyllanthus amarus.Methods: Phenolic contents of the hydromethanolic extracts were measured using Folin-Ciocalteu reagent. Antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl hydrate free radical scavenging and antilipid peroxidation assays. Cytotoxicity on human hepatoma HepG2 cells was assessed by trypan blue and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide viability assays. A stereomicroscope was used to observe and photograph the morphology of the cells. Oxygen consumption of the HepG2 cells was measured using a Clark oxygen electrode. Results: The extract of P. virgatus, which contained more phenolic compounds than P. amarus extract, had higher cytotoxicity and showed higher free radical scavenging activity and more inhibition of peroxidation in a linoleic acid system. P. virgatus extract conspicuously increased the oxygen consumption of HepG2 cells, while P. amarus extract had little stimulatory effect. Conclusion: The hydromethanolic extract of P. virgatus had stronger antioxidant and cytotoxic action than P. amarus extract. The stimulation of HepG2 cell respiration by P. virgatus extract suggests the extract alters mitochondrial function; this action could play a role in the cytotoxicity of this plant. |
| Databáze: | OpenAIRE |
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