Analysis of Urinary 17-Ketosteroids by Gas-Liquid Chromatography

Autor:	Maryon W. Ruchelman, V. William Cole
Rok vydání:	1966
Předmět:	chemistry.chemical_compound Chromatography Trimethylsilyl chemistry Resolution (mass spectrometry) Biochemistry (medical) Clinical Biochemistry Extraction (chemistry) Ether Sulfuric acid Acid hydrolysis Gas chromatography Fractionation
Zdroj:	Clinical Chemistry. 12:771-788
ISSN:	1530-8561 0009-9147
Popis:	A method is presented for the resolution of urinary 17-ketosteroids (17-KS) by gas liquid chromatography (GLC) for clinical application. Hot acid hydrolysis with sulfuric acid is followed by ether extraction, 1 N NaOH wash, and NaOH pellets to serve for both purifying and drying agent. All steps take place in the same reaction tube and are adapted for mechanical shaking. The method allows one analyst to prepare 6-8 urine extracts and trimethylsilyl ether (TMS) derivatives in 1 working day, with GLC the next morning. A quantitative fractionation is routine within 24 hr.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7d05b0a42e97729280cd58cfe1e0e7e8 https://doi.org/10.1093/clinchem/12.11.771 Zobrazit plný text záznamu