| Autor: | Naoki Eisaki, Takaharu Sakiyama, Tsuyoshi Yamanoue, Koshiki Mino, Katsuhiro Ohno, Asahi Matsuyama, Kazuhiro Nakanishi |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Alanine
chemistry.chemical_classification Recombinant escherichia coli biology Bioengineering General Medicine Cysteine synthase biology.organism_classification medicine.disease_cause Applied Microbiology and Biotechnology Molecular biology Enterobacteriaceae Enzyme chemistry biology.protein medicine Serine O-acetyltransferase Escherichia coli Bacteria Biotechnology |
| Zdroj: | Biotechnology Letters. 23:2051-2055 |
| ISSN: | 0141-5492 |
| Popis: | β-(Pyrazol-1-yl)-l-alanine (β-PA) was produced from l-serine and pyrazol using recombinant Escherichia coli cells expressing serine acetyltransferase and O-acetylserine sulfhydrylase-A. The amount of β-PA increased with increasing l-serine concentrations up to 600 mM at 50 mM pyrazol while 100 mM pyrazol gave the highest β-PA production with 50 mM l-serine. Under the optimized conditions, β-PA accumulated in the broth at approximately 140 mM with a conversion of 90% with respect to the added amount of pyrazol. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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