POTENTIALITIES OF ZEOLITES FOR IMMOBILIZATION OF ENZYMES IN CONDUCTOMETRIC BIOSENSORS

Autor:	Basma Khadro, Albert Sacco, Burcu Akata Kurç, Sergei V. Dzyadevych, Esin Soy, Valentyna N. Arkhypova, Viktoriya Pyeshkova, Nicole Jaffrezic-Renault
Rok vydání:	2014
Předmět:	Oxidase test Immobilized enzyme biology Urease technology industry and agriculture chemistry.chemical_compound Membrane chemistry biology.protein Glucose oxidase Glutaraldehyde Zeolite Biosensor Nuclear chemistry
Zdroj:	Sensor Electronics and Microsystem Technologies. 7:28-35
ISSN:	2415-3508 1815-7459
Popis:	Conductometric biosensors based on urease and glucose oxidase immobilized with different types of zeolites have been investigated and compared. For this purpose, zeolite A, zeolite Y, Silicalite-1 (spherical), Silicalite-2, H+Beta 300, H+Beta 150 and NH4+Beta 25 were compared as potential carriers for enzyme immobilization. The parameters to obtain optimized biosensor performance were studied by investigating the percentage of zeolite in membrane, immobilization time in glutaraldehyde vapor and pH of the environment. Different zeolite types resulted in different enzymatic responses. In particular, we have demonstrated that the urease immobilized on silicalite-2 had better performance than immobilized urease without zeolite. Conductometric biosensor with glucose oxidase immobilized with NH4+Beta 25 zeolite had similar response values compared with immobilised enzyme without zeolite. The results obtained show that zeolites could be used as alternatives for enzyme immobilization in conductometric biosensors development.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7cfa311ddc14e7e1a89558186be21819 https://doi.org/10.18524/1815-7459.2010.1.114008 Zobrazit plný text záznamu