A novel LC-MS/MS method for simultaneous quantification of tenofovir and lamivudine in human plasma and its application to a pharmacokinetic study
| Autor: | Seshagiri Rao Jvln, Jaswanth Kumar Inamadugu, Laxminarayana Burugula, Murali K. Matta, Nageswara Rao Pilli |
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| Rok vydání: | 2012 |
| Předmět: |
Pharmacology
Analyte Accuracy and precision Chromatography Formic acid Calibration curve Electrospray ionization Clinical Biochemistry Lamivudine General Medicine Biochemistry Analytical Chemistry chemistry.chemical_compound Pharmacokinetics chemistry Abacavir Drug Discovery medicine Molecular Biology medicine.drug |
| Zdroj: | Biomedical Chromatography. 26:1202-1209 |
| ISSN: | 0269-3879 |
| DOI: | 10.1002/bmc.2679 |
| Popis: | A new, rapid, sensitive and specific LC-MS/MS method has been developed and validated for the simultaneous quantification of tenofovir and lamivudine in human plasma using abacavir as an internal standard. An API-4000 LC-MS/MS with electrospray ionization was operated in multiple-reaction monitoring mode for the analysis. The analytes were extracted from plasma by solid-phase extraction technique using an Oasis HLB cartridge. The reconstituted samples were chromatographed on a Chromolith ROD speed C18 column using a mixture of 0.1% formic acid in water and acetonitrile (90:10 v/v) at a flow-rate of 1 mL/min. The method was validated as per the FDA guidelines. The calibration curves were found to be linear in the range of 5–600 ng/mL for tenofovir and 25– 4000 ng/mL for lamivudine. The intra- and inter-day precision and accuracy results were well within the acceptable limits. A run time of 2.8 min consumed for each sample made it possible to analyze more samples per day. The proposed assay method was found to be applicable to a pharmacokinetic study in human male volunteers. Copyright © 2012 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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