| Popis: |
This study aims at evaluating the antimicrobial effect of Aloe vera and Sodium hypochlorite under varying parameters on coliform isolated from oil polluted water. Presumptive test was carried out on the oil polluted water sample obtained from Ibafon in Apapa, Lagos, Nigeria to determine the presence of coliforms; confirmatory test was done on the positive samples to determine the coliform thermo-tolerant characteristics. A Completed test was carried out to isolate Escherichia coli O157:H7. The oil polluted water shows a coliform count of >1800 cfu/100ml. Thermo-tolerant E. coli (TE), pathogenic E. coli (PE) and non-pathogenic E. coli (NE) were detected and isolated in the water sample. The efficacy of NaOCl and Aloe vera gel as antimicrobials on the isolates were recorded at 5% concentration. NaOCl at 0 and 25°C, pH 10 and 10hr sunlight exposure was active against the isolates. Aloe vera at 25°C, pH 4.0, and zero sunlight exposure time exhibited inhibitory effect on the growth of thermo-tolerant E. coli. Pathogenic E. coli isolate was resistant to aloe vera at all used pH. Aloe vera at 0 and 55°C, pH 6, and 10hr of sunlight exposure time inhibited non-pathogenic E. coli growth. Both Aloe vera and Sodium hypochlorite showed antimicrobial activities towards the coliform isolated in this study and their efficacy were dependent on the conditions at which they were utilized. |
