Effects of destruxins on free calcium and hydrogen ions in insect hemocytes
| Autor: | Shunxiang Ren, Xiao-Qiang Yu, Xiurun Chen, Qiongbo Hu |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
biology
fungi Metarhizium anisopliae Spodoptera litura Bafilomycin chemistry.chemical_element Calcium biology.organism_classification General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Mechanism of action chemistry Cell culture Bombyx mori Insect Science Botany Biophysics medicine medicine.symptom Agronomy and Crop Science Ecology Evolution Behavior and Systematics Intracellular |
| Zdroj: | Insect Science. 21:31-38 |
| ISSN: | 1672-9609 |
| DOI: | 10.1111/1744-7917.12028 |
| Popis: | Destruxins, cyclohexadepsipeptidic mycotoxins isolated from the entomopathogenic fungus Metarhizium anisopliae, inhibit innate insect immunity. However, their mechanism of action remains unclear. In this study, the effects of destruxins on changes in free calcium and hydrogen ions in the hemocytes of Exolontha serrulata, Bombyx mori and the Spodoptera litura SL-1 cell line were detected using laser scanning confocal microscopy (LSCM). An instant Ca(2+) influx of hemocytes induced by destruxins A and B (DA and DB) was recorded. The DA/DB-dependent Ca(2+) influx was not influenced by the Ca(2+) channel inhibitors 2-aminoethoxydiphenyl borane (2-APB) and U73122. It also had an apparently different LSCM profile from that of the ionomycin-dependent Ca(2+) influx. However, the instant Ca(2+) influx was not seen in the SL-1 cells; on the contrary, a slow, moderate enhancement of intracellular Ca(2+) was observed. Meanwhile, an instant intracellular free H(+) decrease aroused by DA and DB was found. DB at 20 μmol/L and DA at 690 μmol/L significantly reduced intracellular free H(+) levels. Furthermore, the vacuolar H(+)-ATPase (V-ATPase) inhibitor bafilomycin A1 had obvious effects on the decreases of intracellular free H(+) in hemocytes. These results suggest that the mechanism of DA/DB-dependent Ca(2+) influx is perhaps not related to Ca(2+) channels and ionophores; rather, the intracellular free H(+) decrease might be due to V-ATPase inhibition. |
| Databáze: | OpenAIRE |
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