Design of an efficient fermentation process for the production of Metarhizium acridum blastospores
| Autor: | J. L. Navarrete-Bolaños, M. G. Téllez-Martínez, G. Alcalá-Gómez, H. Jiménez-Islas |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology business.industry Hypocreales biology.organism_classification 01 natural sciences Corn steep liquor Biotechnology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Insect Science Monopotassium phosphate Bioreactor Blastospore Metarhizium acridum Fermentation Response surface methodology Food science business Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Biocontrol Science and Technology. 26:1652-1667 |
| ISSN: | 1360-0478 0958-3157 |
| DOI: | 10.1080/09583157.2016.1231775 |
| Popis: | Using a sequential approach, we described efficient blastospore production in a stirred tank bioreactor (3 L capacity). We used the response surface methodology to optimise the media ingredients and fermentation parameters to obtain the maximum production of blastospores by a locally collected isolate of Metarhizium acridum (Ascomycota: Hypocreales). The results showed that a liquid culture medium supplemented with monopotassium phosphate (15.17 g/L), corn steep liquor (69.25 g/L), and casamino acids (80.68 g/L) in a stirred tank bioreactor under operating conditions constant at 635 rpm, a temperature of 26°C, and pH 3.3 produced 1.25 × 108 blastospores (bls)/ml, with 93% viability after 120 h of fermentation. This bioreactor yield compares favourably with the yields obtained by shake flask production and confirms the suitability of the media and production parameters for the potential scale-up fermentation production of M. acridum. |
| Databáze: | OpenAIRE |
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