The Genetic Relationship Between Mycoplasma-like Organisms Causing Diseases in the Sudan and Different Continents Revealed by Polymerase Chain Reaction (PCR) Amplification of the 16S rRNA Gene Followed by Restriction Fragment Length Polymorphism Analysis
| Autor: | Essam M. Saeed, Marie-Thérèse Cousin |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
0106 biological sciences
2. Zero hunger Genetics 0303 health sciences 030306 microbiology Physiology food and beverages Plant Science Biology 01 natural sciences Vicia faba 03 medical and health sciences Restriction enzyme Prunus Genetic marker Plant virus Botany Phyllody Restriction fragment length polymorphism Agronomy and Crop Science Ribosomal DNA 010606 plant biology & botany |
| Zdroj: | Journal of Phytopathology. 143:17-20 |
| ISSN: | 1439-0434 0931-1785 |
| DOI: | 10.1111/j.1439-0434.1995.tb00193.x |
| Popis: | The genetic relationship between faba bean (Vicia faba L.) phyllody and other mycoplasma-like organism (MLO) diseases has been studied by amplification of the conserved region of the 16S rRNA gene followed by restriction fragment length polymorphism (RFLP) analysis using Alu I restriction endonuclease. The restriction patterns produced by faba bean phyllody MLO were smilar to that of Crotalaria saltiana phyllody MLO which persists throughout the year in the Sudan. These, and serological results clearly confirmed that C. saltiana is a reservoir of faba bean phyllody MLO in the Sudan. Moreover, restriction patterns have also shown that MLOs of other diseases have the same RFLP fragment pattern as faba bean phyllody MLO, including C. juncea witches'broom (Thailand) and tomato big-bud (Australia), which differs from the other selected MLO diseases (Gladiolus aster yellow, clover phyllody and yellow decline of lavender, aqll from France). Fragment patterns also revealed the existence of genetically diverse MLO strains in the Sudan. Faba bean phyllody may be placed in group III including WX, apricot chlorotic leaf roll, golden flaveswcence doree of grapevine, plum leptonecrosis of Prunus salciana, peachy yellow leaf roll, sunnhemp phyllody from Thailand, and blueberry witches' broom. |
| Databáze: | OpenAIRE |
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