| Autor: |
Matthias Mann, Andreas-David Brunner, Maximilian T. Strauss, Fabian J. Theis, Eugenia Voytik, Florian Meier, Wanka Jh, Niklas Köhler |
| Rok vydání: |
2020 |
| Předmět: |
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| Popis: |
The size and shape of peptide ions in the gas phase are an under-explored dimension for mass spectrometry-based proteomics. To explore the nature and utility of the entire peptide collisional cross section (CCS) space, we measure more than a million data points from whole-proteome digests of five organisms with trapped ion mobility spectrometry (TIMS) and parallel accumulation – serial fragmentation (PASEF). The scale and precision (CV 0.99). Hydrophobicity, position of prolines and histidines are main determinants of the cross sections in addition to sequence-specific interactions. CCS values can now be predicted for any peptide and organism, forming a basis for advanced proteomics workflows that make full use of the additional information. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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