| Popis: |
Intraperitoneal inoculation (IP) is the most effective parenteral route for priming a respiratory immune response. We initiated tracer studies to define the distribution of both colloidal carbon and fluorescent microbeads injected IP. We observed, in addition to lymphatic drainage of the carbon, migration of macrophages containing carbon from the peritoneal cavity directly to the lung interstitium and bronchial associated lymphatic tissue (BALT) without passage in the blood. FACS analysis of migrant phagocytic peritoneal macrophages indicated that that they bear a phenotype (Mac1+, Ia+ and L3T4+) consistent with antigen presenting cells. This suggests that peritoneal macrophages are a source of lung interstitial macrophages which could present antigen to immunocompetent mucosa-associated lymphoid cells in the lung following IP immunization. We propose that IP immunization results in simultaneous priming of both the systemic and mucosal immune response. |
