Interferon-α neutralizing antibodies in HIV and chronic HCV patients treated with natural-source human leukocyte-derived interferon-αn3

Autor:	Teresa Smith, Katalin Ferencz-Biro, Xiao-Xia Zhao, Mei-June Liao, Abbas Rashidbaigi, Ji Hua
Rok vydání:	1997
Předmět:	biology business.industry medicine.medical_treatment Immunology Antibody titer Alpha interferon General Medicine Immunotherapy Virology Virus Titer Interferon medicine biology.protein Immunology and Allergy Antibody business Interferon alfa medicine.drug
Zdroj:	Human Antibodies. 8:129-136
ISSN:	1875-869X 1093-2607
DOI:	10.3233/hab-1997-8304
Popis:	Human leukocyte-derived IFN-alpha n3 (Alferon N Injection) was administered subcutaneously to treat 20 patients with asymptomatic human immunodeficiency virus type 1 (HIV-1) and 141 patients with chronic hepatitis C virus (HCV) infections. The treatment of HIV-1 and HCV patients, previously untreated with any IFN preparations, did not result in development of neutralizing antibodies to IFN-alpha n3. Among 69 HCV refractory patients who were unresponsive to previous treatment with rIFN-alpha 2b, 2 had neutralizing antibodies to rIFN-alpha 2b prior to IFN-alpha n3 therapy, with no or limited cross-reactivity to IFN-alpha n3. After retreatment with IFN-alpha n3, both patients had detectable neutralizing titers to IFN-alpha n3. Additionally, 2 other patients developed low and transient neutralizing titers to IFN-alpha n3. Interferon subtype specificity of these antibodies was tested against RP-HPLC purified fractions of IFN-alpha n3, as well as rIFN-alpha 2b and rIFN-alpha 8b. Sera from patients previously treated with rIFN-alpha 2b with high antibody titers to rIFN-alpha 2b strongly reacted with the natural IFN-alpha 2b, and to a limited extent with other iFN-alpha subtypes. Neutralizing activity against IFN-alpha 2b was significantly competed out by the presence of a small amount of other interferon subtypes present in IFN-alpha n3. One patient with prior presence of antibodies to IFN-alpha 2b developed a high antibody titer to IFN-alpha 8b with limited reactivity to IFN-alpha n3. Two of the HCV refractory patients with prior neutralizing antibodies to rIFN-alpha 2b responded to IFN-alpha n3 therapy. These data suggest that the presence of neutralizing antibodies to individual IFN-alpha species will not significantly diminish the biological activity and the clinical efficacy of multi-species IFN-alpha n3.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7c6de4ead958bd1bf2a34f9a7508586a https://doi.org/10.3233/hab-1997-8304 Zobrazit plný text záznamu