Identification and localization of calcium-dependent protease II inNeurospora crassa andUromyces appendiculatus

Autor:	Y. Jaquet, Mukti Ojha, R. Ortega Perez, Francisco Barja, Harvey C. Hoch
Rok vydání:	1999
Předmět:	chemistry.chemical_classification Hyphal growth Hypha Molecular mass medicine.diagnostic_test fungi Crassa Cell Biology Plant Science General Medicine Biology Immunofluorescence biology.organism_classification Enzyme chemistry Biochemistry Western blot medicine Calcium dependent protease
Zdroj:	Protoplasma. 210:85-91
ISSN:	1615-6102 0033-183X
DOI:	10.1007/bf01314958
Popis:	The existence of Ca2+-dependent protease II in crude extracts ofNeurospora crassa andUromyces appendiculatus was demonstrated by immunoblotting using specific antibodies. In both extracts two immunoreacting bands were observed. The molecular mass of the major band inN. crassa corresponded to 37 kDa, while that inU. appendiculatus was 43 kDa, similar to that previously reported forAllomyces arbuscula. Immunofluorescence of the enzyme was predominantly localized in the apical regions of germlings and growing hyphae, suggesting a functional role for the enzyme in hyphal growth.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7c50b1f3f201512c50a4b9db2a64db9c https://doi.org/10.1007/bf01314958 Zobrazit plný text záznamu Full text from SpringerLink