Photorespiration and Malate Formation in Grape Leaves

Autor: U. Malipiero, H.P. Ruffner, Dora M. Rast
Rok vydání: 1981
Předmět:
Zdroj: Zeitschrift für Pflanzenphysiologie. 104:243-251
ISSN: 0044-328X
DOI: 10.1016/s0044-328x(81)80118-3
Popis: Summary Excised grape leaves were fed 14 CO 2 in a closed assimilation chamber for 10 and 30 min, respectively, and the incorporation of radioactive label into the watersoluble substances was determined. Under the experimental conditions glycine/serine and sucrose constituted the main radioactive pools, whilst only traces of label were detectable in glycolate. However, administration of glycolate oxidase inhibitors (HPMS or GBS) through the leaf petiole prior to 14 CO 2 labelling led to an accumulation of 14 C-glycolate, thus indicating that in grape leaves a considerable portion of fixed carbon is normally metabolized via the photorespiratory pathway. Environmental conditions which favour photorespiration caused enhanced incorporation of 14 CO 2 not only into glycolate pathway intermediates but also into glycerate and malate. This finding suggests a close precursor-product relationship between the relevant photorespiratory compounds on the one hand and glycolytic C 3 -compounds and malic acid on the other.
Databáze: OpenAIRE