Probing tumor and peritumoral tissues in superficial and nodular basal cell carcinoma using polarized Raman microspectroscopy
| Autor: | Philippe Bernard, Anne Durlach, Michel Manfait, Frank Antonicelli, Olivier Piot, Elodie Ly |
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| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Experimental Dermatology. 19:68-73 |
| ISSN: | 1600-0625 0906-6705 |
| DOI: | 10.1111/j.1600-0625.2009.00992.x |
| Popis: | Basal cell carcinoma (BCC) can sometimes lead, through a possible invasion of the dermis and the subcutaneous tissue, to serious local damage to the patient. Several histological types of BCC are reported, among them, the superficial, nodular and infiltrative forms. This study reports the use of polarized Raman microspectroscopy on the nodular and superficial types to discriminate between healthy epidermis and tumor, and between normal and peritumoral stroma. This technique probes additional information than conventional Raman spectroscopy because it is sensitive to the molecular ordering of tissue components. Depolarization ratios and hierarchical cluster analysis demonstrate that polarized Raman microspectroscopy can better identify the tumor and the peritumoral dermis than conventional Raman microspectroscopy, and hence gives potential complementary data about their molecular characteristics (molecular composition, secondary structure of proteins, intra- and/or inter-molecular bonding). Our findings also show that although superficial and nodular types of BCC were analysed, clear differences between the spectra of peritumoral and normal dermis could be detected. |
| Databáze: | OpenAIRE |
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