Hyperosmotic stress-induced somatic embryogenesis and its continuous culture in Japanese honewort (Cryptotaenia japonica)
| Autor: | Mugito Kato, Hajime Shiota |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0303 health sciences Sucrose Somatic embryogenesis Osmotic shock food and beverages Embryo Plant Science Biology Cryptotaenia japonica biology.organism_classification 01 natural sciences Japonica 03 medical and health sciences Horticulture chemistry.chemical_compound chemistry Germination Agronomy and Crop Science 030304 developmental biology 010606 plant biology & botany Biotechnology Explant culture |
| Zdroj: | Plant Biotechnology. 38:31-36 |
| ISSN: | 1347-6114 1342-4580 |
| DOI: | 10.5511/plantbiotechnology.20.0910a |
| Popis: | Japanese honewort (Cryptotaenia japonica) is consumed as a traditional vegetable and has medicinal applications. In Japan, C. japonica is mainly produced using hydroponic culture systems; however, damping-off is often caused by the adherence of pathogens to its seeds. Therefore, the use of sterile artificial seeds in hydroponic culture is likely to be effective for preventing disease. In this study, we established methods for stress-induced somatic embryogenesis and artificial seed production in Japanese honewort. Shoot apex explants from seedlings were treated with 0.7 M sucrose as a hyperosmotic stress for 3 or 6 weeks, and then transferred to stress-free conditions. Somatic embryos were formed after culture in stress-free conditions for 7 weeks. Stress-treated shoot apex explants that formed somatic embryos were cultured in Murashige and Skoog liquid medium with shaking. After 2 weeks of culture, approximately 800 somatic embryos were formed from each explant. Somatic embryos were formed continuously during 37 weeks under the same culture conditions. Thus, somatic embryogenesis was effectively induced in Japanese honewort via hyperosmotic stress, and embryogenic competence was maintained under stress- and phytohormone-free conditions. The somatic embryos produced by liquid culture were used to produce artificial seeds by enveloping the embryos in whipped alginate gel to avoid hypoxic conditions. The artificial seeds had a high germination rate (72%). This system is suitable for the sterile, highly productive hydroponic culture of Japanese honewort. |
| Databáze: | OpenAIRE |
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