Anatomical and histochemical characterization of in vitro haustorium from roots of Castilleja tenuiflora

Autor:	Francisco Cruz-Sosa, Antonio Ruperto Jiménez-Aparicio, Guadalupe Salcedo-Morales, Gabriela Trejo-Tapia
Rok vydání:	2014
Předmět:	Vanillin food and beverages Catechin Plant Science Horticulture Biology In vitro chemistry.chemical_compound Biochemistry chemistry Castilleja tenuiflora Haustorium Inducer Elongation
Zdroj:	Biologia plantarum. 58:164-168
ISSN:	1573-8264 0006-3134
Popis:	In vitro induction of haustoria from Castilleja tenuiflora Benth. was achieved by applying 25 μM catechin, 25 μM vanillin, or 25 μM H2O2. Of the treatments tested, 25 μM vanillin was the strongest inducer of haustoria in C. tenuiflora roots in vitro (up to 3 haustoria per root). Haustorium development occurred laterally and was observable 14 d after inducer application. It was characterized by elongation of the epidermal cells and division of the inner cortical cells which also possessed abundant nuclei. Histochemical analysis using 3,3-diaminobenzidine (DAB) and diphenylboric acid 2-aminoethyl ester (DBPA) indicated that the formation of haustoria was associated with the accumulation of H2O2 and flavonoids.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7c3999e2344d8859866ebd00df5a75d0 https://doi.org/10.1007/s10535-013-0369-2 Zobrazit plný text záznamu Full text from SpringerLink