Abstract A43: Investigation of dipeptidyl peptidase IV/CD26 role in cervical cancer cell lines
| Autor: | Juliano D. Paccez, Danielle Bertodo Santana, Márcia Rosangela Wink, Alessandra Nejar Bruno, Luiz F. Zerbini, Andréia Buffon, Jéssica Nascimento, Aline Beckenkamp |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Molecular Cancer Therapeutics. 12:A43-A43 |
| ISSN: | 1538-8514 1535-7163 |
| DOI: | 10.1158/1535-7163.targ-13-a43 |
| Popis: | Cervical cancer is a common type of cancer in women and an important public health problem, mainly in developing countries. The glycoprotein DPPIV/CD26 is expressed at the membrane of many cells. It is able to inactivate some bioactive peptides and chemokines, thereby regulating cell proliferation and survival, being involved in processes related to cancer. This enzyme is also present in a soluble form (DPPIV/sCD26), in biological fluids. In this study, we evaluated, the expression and enzymatic properties of the DPPIV/CD26 and its relation with tumoral mechanisms in cervical cancer cell lines. The cervical cancer cells exhibit DPPIV/CD26 enzymatic activity in both membrane bound and, its soluble form, which was confirmed by the inhibition of the enzymatic DPPIV/CD26 activity for sitagliptin phosphate. The CD26 mRNA expression was observed in the SiHa and C33A cells, while the HeLa cells showed no transcripts. The DPPIV/CD26 expression was correlated with the enzymatic activity in these cell lines. We also observed a higher migratory capacity of HeLa (cervical cancer cell line that less express CD26) compared to SiHa (cervical cancer cell line that more express CD26), moreover in the presence of the specific inhibitor we observed that SiHa cell line shows an increase in migration, thus confirming a relationship of this enzyme with the migratory ability. Our results show, that cervical cancer cells present DPPIV/CD26 enzymatic activity in both membrane bound form, as in its soluble form, revealing a differential expression as well as its relationship to cell migration and adhesion. Considering that DPPIV/CD26 plays an important role in tumor biology, this protein may become an important therapeutic target although additional work is required to elucidate the molecular mechanisms associated with DPPIV/CD26 in cervical cancer. Citation Information: Mol Cancer Ther 2013;12(11 Suppl):A43. Citation Format: Andréia Buffon, Aline Beckenkamp, Danielle Bertodo Santana, Jéssica Nascimento, Juliano Paccez, Luiz Fernando Zerbini, Márcia Rosangela Wink, Alessandra Nejar Bruno. Investigation of dipeptidyl peptidase IV/CD26 role in cervical cancer cell lines. [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; 2013 Oct 19-23; Boston, MA. Philadelphia (PA): AACR; Mol Cancer Ther 2013;12(11 Suppl):Abstract nr A43. |
| Databáze: | OpenAIRE |
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