| Popis: |
Several experimental designs require serial dilutions of site water. To perform dilutions without the use of prefiltered water or without the addition of nutrients and trace metals in freshwater systems, major ion solutions (MIS) are required. MIS provides a nutrient and metal free dilution medium to minimize hypertonic and hypotonic effects on the organisms in the samples during dilutions by balancing major dissolved ions of Ca2+, Mg2+, Na+, K+, Cl-, SO42-, and Si4+ in freshwater systems. MIS are the equivalent of no nutrient artificial sea water for use in freshwater systems. Unlike artificial sea water, MIS formulation for use in freshwater systems changes from site to site. This protocol describes the process of creating a site specific major ion solution from results of elemental analysis data for use in the specified freshwater system. |
