Photoinduced Immobilization of Biomolecules on the Surface of Azopolymer Films and Its Dependence on the Concentration and Type of the Azobenzene Moiety

Autor:	Makoto Mouri, Osamu Watanabe, Masaaki Tsuchimori, Mamiko Narita, Taiji Ikawa, Fumihiko Hoshino
Rok vydání:	2007
Předmět:	Acrylate Azo compound Aqueous solution Polymers and Plastics Organic Chemistry Inorganic Chemistry chemistry.chemical_compound chemistry Azobenzene Polymer chemistry Materials Chemistry Copolymer Side chain Moiety Visible spectrum
Zdroj:	Macromolecules. 40:623-629
ISSN:	1520-5835 0024-9297
DOI:	10.1021/ma061601z
Popis:	We report on the photoinduced immobilization of Immunoglobulin G (IgG) on the surface of azobenzene-bearing acrylate copolymers (azopolymers). Two different types of azopolymers were synthesized that incorporated either a 4-aminoazobenzene moiety (H-azopolymer) or a 4-aminocyanoazobenzene moiety (CN-azopolymer), using different concentrations of the respective moieties. IgG was immobilized on the surfaces of the azopolymer films by exposure to visible light, and each of the films was then treated with an aqueous solution of an antigen. Antigen−antibody reactions were confirmed on the surfaces of the films, indicating that immobilized IgG generated by a photoirradiation can retain its activity. The amount of the immobilized antibodies on the azopolymer surfaces increased with azobenzene content up to 30 wt % and saturated over 30 wt % when measured under the same conditions. The efficiency of the immobilization process was found to correlate with the depth of deformations on the surfaces of the azopolymers...
Databáze:	OpenAIRE
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