A miniaturized, DNA-FET biosensor-based microfluidic system for quantification of two breast cancer biomarkers
| Autor: | Po-Chiun Huang, Gwo-Bin Lee, Yi-Sin Chen, Yu-Hsuan Kuo, Chi-Chien Huang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
business.industry
010401 analytical chemistry Microfluidics Improved survival 02 engineering and technology Extracellular vesicle 021001 nanoscience & nanotechnology Condensed Matter Physics medicine.disease 01 natural sciences 0104 chemical sciences Electronic Optical and Magnetic Materials Highly sensitive Breast cancer Materials Chemistry Cancer research medicine Biomarker (medicine) 0210 nano-technology business Biosensor |
| Zdroj: | Microfluidics and Nanofluidics. 25 |
| ISSN: | 1613-4990 1613-4982 |
| Popis: | Breast cancer is among the frequently diagnosed cancers worldwide and is associated with a high mortality rate, especially when diagnosed late. Minimally invasive screening approaches based on an assessment of extracellular vesicle (EV)-encapsulating microRNA biomarkers have enabled earlier diagnosis and improved survival rates. Since field-effective transistors (FET) featuring complementary metal oxide semiconductor technology have been previously converted into highly sensitive biosensors, an integrated microfluidic system (IMS) was developed herein for quantifying concentrations of breast cancer biomarkers including microRNA-195 and microRNA-126. Following a (1) 4-h process in which 84% of the EVs were captured, (2) 20-min hybridization step in which 85 and 94% of the microRNA-195 and microRNA-126 were isolated, respectively, and (3) the DNA-FET biosensors could detect down to 84 and 75 aM concentrations of microRNA-195 and microRNA-126, respectively. The IMS automated the entire biomarker quantification process within 5 h, highlighting its potential as a sensitive platform for early-stage breast cancer diagnosis. |
| Databáze: | OpenAIRE |
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