Flower-bud failure in olive and the involvement of amoeboid protists

Autor: C. Medeira, I. Maia, T. Carvalho
Rok vydání: 2006
Předmět:
Zdroj: The Journal of Horticultural Science and Biotechnology. 81:251-258
ISSN: 2380-4084
1462-0316
DOI: 10.1080/14620316.2006.11512058
Popis: SummaryFlower bud anomalies in Olea europaea L., ‘Santulhana’ and ‘Conserva de Elvas’ begin as very small microscopic lesions associated with amoeboid bionts, which cause extensive areas of destruction and block flower organ development. In this work, we describe the process of injury in olive flower-buds in parallel with biont development in floral tissues and in the culture medium. Flower-buds were processed for light microscopy (LM) and transmission electron microscopy (TEM). Bionts were isolated from flower-buds in culture medium and processed for LM. Amoeboid bionts in several stages of plasmodial formation, grown in culture medium from olive flower-buds, resembled those observed in sectioned flower-bud tissues. The formation of small plasmodia by the fusion of amoeboid cells and the development of small plasmodia into phaneroplasmodia, suggest the presence of an amoeboid protist of the Myxogastria group. On the other hand, sporulation of plasmodia in hay agar culture medium, gave rise to stalked spo...
Databáze: OpenAIRE