Teaching Undergraduates LC–MS/MS Theory and Operation via Multiple Reaction Monitoring (MRM) Method Development
| Autor: | Julie A. Palkendo, Thomas A. Betts |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Chromatography
Materials science Vanillin 010401 analytical chemistry 05 social sciences Selected reaction monitoring 050301 education General Chemistry Mass spectrometry 01 natural sciences Method development 0104 chemical sciences Education Triple quadrupole mass spectrometer chemistry.chemical_compound chemistry Lc ms ms Ethyl vanillin Laboratory experiment 0503 education |
| Zdroj: | Journal of Chemical Education. 95:1035-1039 |
| ISSN: | 1938-1328 0021-9584 |
| DOI: | 10.1021/acs.jchemed.7b00914 |
| Popis: | This laboratory experiment employs an innovative approach to introduce undergraduates to liquid chromatography–tandem mass spectrometry (LC–MS/MS) by developing a method based on multiple reaction monitoring (MRM). During this qualitative investigation, students operated a triple quadrupole mass spectrometer in the MS scan and product ion scan modes to determine precursor and product ions, respectively, for a vanillin, ethyl vanillin, and coumarin standard mixture. After identifying precursor → product ion transitions, students conducted a 2 min liquid chromatography run in MRM mode. Students were astonished by the speed, selectivity, and sensitivity of the LC–MS/MS method compared to that of a liquid chromatograph with ultraviolet detection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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