Evidence of Systemic Responses to Viral Pathogens using Malondialdehyde, Tumor Necrosis Factor Alpha and Superoxide Dismutase
| Autor: | Mathew Folaranmi Olaniyan, Tolulope Busayo Ojediran, Gbenga Shedrack Olayinka |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
biology business.industry virus diseases Inflammation Venous blood medicine.disease_cause Malondialdehyde 030226 pharmacology & pharmacy Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry Immunology medicine biology.protein Sputum Ziehl–Neelsen stain Tumor necrosis factor alpha medicine.symptom business Oxidative stress |
| Zdroj: | International Journal of Clinical and Experimental Physiology. 7:18-21 |
| ISSN: | 2348-8093 2348-8832 |
| Popis: | Background and Aim: Infectious agents are triggers of oxidative stress and inflammation. This work was designed to determine evidence of systemic responses to viral pathogens using malondialdehyde, tumor necrosis factor alpha and superoxide dismutase. Methods: The subjects recruited for this study include 23 HCV mono-infected volunteers (aged 24-67 years; Male-10; Female-13), 30 HBV mono-infected volunteers (aged 24-67 years; Male-15; Female-15), 18 HIV mono-infected volunteers (aged 24-67 years; Male-8; Female-10), 23 Viral (HCV, HIV, HBV) Co-infected volunteers (aged 24-67 years; Male-10; Female-13) and 50 age matched control volunteers who were not infected with HCV, HIV or HBV (Female-25; Male-25). Subjects tested negative to Acid Fast Bacilli (AFB) and Plasmodium tests were recruited. Test subjects who have not initiated antiviral therapy were recruited for the study. Five milliliters of venous blood was obtained from each of the subjects into lithium heparinized bottles for the assay of HCV, HIV, HBV, TNFα by ELISA, SOD, MDA by Colorimetry, Giemsa thick blood film staining for plasmodium while sputum samples were obtained from each of the subjects for Ziehl Neelsen staining for Acid Fast Bacilli(AFB). Results: There was a significant increase in plasma MDA and TNF α in patients with viral co-infection compared with control and patients with mono-infections of HCV, HBV and HIV (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|