| Autor: |
A.A. Bayev, A.D. Mirzabekov |
| Rok vydání: |
1974 |
| Předmět: |
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| DOI: |
10.1016/0076-6879(74)29056-6 |
| Popis: |
Publisher Summary The principle and the technique of the dissected molecule method were first described in 1967. The enzymatic aminoacylation of fragments of Valine tRNA I (baker's yeast) and their aggregates have been studied for the elucidation of the structural basis of tRNA recognition by its cognate aminoacyl-tRNA synthetase. The dissected-molecule method is commonly employed and applied for the study of recognition sites of other tRNA's. As far as tRNA's represent a uniform group of molecules with similar structure and function one may expect that the dissected molecule method can be used for the investigation of this problem in every tRNA. The chapter describes the various experimental procedures utilized for the study of recognition sites in Valine tRNA I by the dissected molecule method: fragment preparation, tests for fragment association, and the assay of the acceptor activity. The chapter discusses the preparation of Valine tRNA I fragments because of its significance for the method. A synopsis of the studies of Valine tRNA I recognition sites is also presented in the chapter. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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