Effect of two crosslinking methods on the physicochemical and biological properties of the collagen-chitosan scaffolds
Autor: | Verónica Mata-Haro, Juan V. Cauich-Rodríguez, Wilberth Herrera-Kao, Víctor A. Reyna-Urrutia, José M. Cervantes-Uc |
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Rok vydání: | 2019 |
Předmět: |
Morphology (linguistics)
Polymers and Plastics Biocompatibility Organic Chemistry technology industry and agriculture General Physics and Astronomy macromolecular substances 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Chitosan chemistry.chemical_compound Ammonium hydroxide Tissue engineering chemistry Chemical engineering Self-healing hydrogels Materials Chemistry Acetone Glutaraldehyde 0210 nano-technology |
Zdroj: | European Polymer Journal. 117:424-433 |
ISSN: | 0014-3057 |
DOI: | 10.1016/j.eurpolymj.2019.05.010 |
Popis: | Chemical and physical crosslinking methods using glutaraldehyde (GA) and ammonium hydroxide (AH), respectively, were utilized to prepare chitosan (CS) and chitosan/collagen (CS-Co) hydrogels; these materials were then subjected to freeze-drying process to obtain 3D porous scaffolds. The effect of crosslinking methods on the physicochemical and biological properties of the scaffolds was studied. Results indicated that crosslinking method plays a key role on morphology, average pore size, pore size distribution, degradation and cytotoxicity of the scaffolds. Physically crosslinked scaffolds exhibited a homogeneous morphology with higher pore size and interconnectivity in comparison to other prepared scaffolds; also, these samples, showed a good biocompatibility. Scaffolds derived from hydrogels treated with acetone (AC) showed shrinkage, smaller pore size and higher degradation rates; finally, materials chemically crosslinked with glutaraldehyde presented cytotoxicity and exhibited a heterogeneous morphology. Overall, these results suggest that the scaffolds physically cross-linked by means of AH, containing or not collagen (CS-AH and CS-Co-AH), are suitable for tissue engineering. |
Databáze: | OpenAIRE |
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