Knockdown of VvMYBA1 via virus-induced gene silencing decreases anthocyanin biosynthesis in grape berries
| Autor: | Peng Fei Wen, Zhang Xiaojun, Gao Yan, Yan Mei Dong, Pengfei Zhang, Tie Quan Niu, Chang Mei Liang, Hao Yu Wen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Wine 0303 health sciences Gene knockdown Transgene fungi food and beverages Virus induced gene silencing (VIGS) Plant Science Horticulture Biology 01 natural sciences Cell biology 03 medical and health sciences chemistry.chemical_compound chemistry Virus-induced gene silencing Anthocyanin Anthocyanin biosynthesis Agronomy and Crop Science 030304 developmental biology 010606 plant biology & botany Regulator gene |
| Zdroj: | Canadian Journal of Plant Science. 100:175-184 |
| ISSN: | 1918-1833 0008-4220 |
| Popis: | Anthocyanin in grapevines, regulated by structural and regulatory genes, determines the colour of grape berries and is a key factor in wine quality. The transgenic approach is a useful strategy for elucidating gene function. However, it is difficult to obtain transgenic fruit trees, including grapevines. Viral vectors offer an effective strategy for overcoming this challenge. In this study, we successfully knocked down the VvMYBA1 gene in ‘Red Globe’ and ‘JiZaoMi’ grape berries via virus-induced gene silencing (VIGS) by vacuum infiltration and silencing efficiencies of 73% and 93%, respectively, were obtained relative to the control. Total anthocyanins content was reduced 1.7- and 2.7-times relative to that in the control in ‘Red Globe’ and ‘JiZaoMi’ grape berries, respectively. The expression levels of VvUFGT and VvDFR were reduced by 80% and 8% and by 72% and 42% relative to the control in ‘Red Globe’ and ‘JiZaoMi’ grape berries, respectively, in the anthocyanin metabolic pathways. Tobacco rattle virus-mediated VIGS was, therefore, successfully established in grape berries. These findings provide direct evidence that VvMYBA1 encodes skin colour in red grape berries and suggest a possible application of VIGS for gene function studies in other fruits. |
| Databáze: | OpenAIRE |
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