Slow growth phenotype - A possible approach to improved plasmid maintenance in Saccharomyces cerevisiae

Autor:	John W. Patching, Ronan D. O'Kennedy
Rok vydání:	1996
Předmět:	biology Strain (chemistry) Saccharomyces cerevisiae Bioengineering General Medicine Chemostat biology.organism_classification Applied Microbiology and Biotechnology Saccharomyces Yeast law.invention Plasmid maintenance Microbiology Plasmid law Recombinant DNA Biotechnology
Zdroj:	Biotechnology Letters. 18:713-718
ISSN:	1573-6776 0141-5492
DOI:	10.1007/bf00130771
Popis:	Transformation-induced slow growth phenotype (SGP) in yeast is repressed in the presence of 2μm plasmids. A full 2μm-sequence-based recombinant plasmid (pJB502) was found to be more stable in a 2μm-free- [cir∘] strain of Saccharomyces cerevisiae than in a cir+ strain. This could not be attributed to differences in growth rate calculated from kinetic analysis of plasmid loss, but transformed [cir∘] isolates, which had lost the recombinant plasmid, exhibited varying degrees of SGP in batch culture. One of these isolates was outcompeted in chemostat culture by the recombinant-plasmid-containing strain, suggesting that improved plasmid maintenance can result from SGP in cir∘ hosts.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7bb9a4868f07dd23c17c26b059e329f7 https://doi.org/10.1007/bf00130771 Zobrazit plný text záznamu Full text from SpringerLink